Numerous studies on fibrosis have been published based on rodent models both in vivo and in vitro. However, notable differences exist between rodents and humans, especially with HSC markers. For example, the “gold standard” marker identified in rodent liver, desmin, is not always expressed in human livers.1 Desmin was found to be negative in normal liver and positive in cirrhotic human livers in some studies, and contrary results were also reported in other studies.1
A system to bridge the gap between rodents and humans is essential for HSC research and drug discovery. Primary isolated HSC from human livers provide researchers a new tool for in vitro studies, which is more applicable to human physiology.
LifeNet Health provides HSC isolated from both healthy and diseased human livers. Our large inventory of HSC with different medical histories provides researchers a large candidate pool from which to select significant differences in donor background.
Authored by Huimin Yan, Ph.D. and Joel LeCluyse