All Human Hepatic Tri-Culture System
LifeNet Health LifeSciences’ Hepatic Tri-Culture Model is currently in product development. This in vitro assay system, comprised entirely of primary human cells: hepatocytes, endothelial, and stromal cells from donated human tissues. This system extends the viability, functionality, and metabolic capacity of primary human hepatocytes for at least four weeks. The cell populations are cultured collectively and allowed to self-assemble into hepatocyte cords, representative of native tissue. The tri-culture platform is ideal for drug discovery and development applications, including metabolic clearance, induction, drug-drug interactions, drug-related hepatotoxicities, and disease modeling of non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), and hepatitis infections.
Tri-Culture System at Day 28: A) Confocal microscopy, B) Staining for different cell types – hepatocytes (red staining depicts albumin), stromal cells (depicted by only blue stain [DAPI] for nuclei), and endothelial cells (green staining with CD31)
Tri-Culture system showing bile canaliculi formation at A) 4X and B) 10X
Tri-Culture system at Day 14 stained for: A) tight junctions with marker ZO-1 and B) gap junctions with marker CX-32