Innovation Pipeline

Tri-Culture

All Human Hepatic Tri-Culture System

Schematic of LifeNet Health LifeSciences all-human Hepatic Tri-Culture system depicting the culture and self assembly of the cell populations into hepatocyte cords, representative of native tissue.

LifeNet Health LifeSciences’ Hepatic Tri-Culture Model is currently in product development. This in vitro assay system, comprised entirely of primary human cells: hepatocytes, endothelial, and stromal cells from donated human tissues. This system extends the viability, functionality, and metabolic capacity of primary human hepatocytes for at least four weeks. The cell populations are cultured collectively and allowed to self-assemble into hepatocyte cords, representative of native tissue. The tri-culture platform is ideal for drug discovery and development applications, including metabolic clearance, induction, drug-drug interactions, drug-related hepatotoxicities, and disease modeling of non-alcoholic fatty liver disease (NAFLD), non-alcoholic steatohepatitis (NASH), and hepatitis infections.

 

Confocal image of LifeNet Health LifeSciences all-human Hepatic Tri-Culture system at day 28
Immunofluorescent image of LifeNet Health LifeSciences all-human Hepatic Tri-Culture system at day 28 stained to show each cell type. Hepatocytes are stained in red to show albumin production, stromal cell nuceli are stained blue with DAPI, and the endothelial cells are stained for CD31 in green.

Tri-Culture System at Day 28: A) Confocal microscopy, B) Staining for different cell types – hepatocytes (red staining depicts albumin), stromal cells (depicted by only blue stain [DAPI] for nuclei), and endothelial cells (green staining with CD31)

LifeNet Health LifeSciences all-human Hepatic Tri-Culture system stained to show bile canaliculi formation at 40X Magnification
LifeNet Health LifeSciences all-human Hepatic Tri-Culture system stained to show bile canaliculi formation at 100X Magnification

Tri-Culture system showing bile canaliculi formation at A) 4X and B) 10X

 Immunofluorescent image of LifeNet Health LifeSciences all-human Hepatic Tri-Culture system at day 14 stained with marker ZO-1 for tight junctions
Immunofluorescent image of LifeNet Health LifeSciences all-human Hepatic Tri-Culture system at day 14 stained with marker CX-32 for gap junctions

Tri-Culture system at Day 14 stained for: A) tight junctions with marker ZO-1 and B) gap junctions with marker CX-32

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